Active substances: Ciprofloxacin
All other chemicals were obtained from Sigma-Aldrich.
Ussing Chamber Experiments. Human tissue samples were collected from three such male patients with their informed consent.
None of the patients had undergone radiotherapy or chemotherapy before surgery. Blood perfusion of the intestine was maintained until the moment of excision. Approximately 10 cm of proximal jejunum was removed and immediately placed in ice-cold Krebs bicarbonate-Ringer solution, presaturated with carbogen.
The tissue was then transported from the hospital operating theater to the laboratories at AstraZeneca Macclesfield, Cheshire, UK.
Tissue was prepared for use in the Ussing chambers as described previously Haslam et al.
Male Han Wistar rats Harlan, UK, aged approximately 100 days and weighing 250 to 400 g, were used in the experiments.
After abdominal incisions, intestinal lumens were occluded by thin cotton thread proximal to the sections of tissue taken from duodenum within 15 cm proximal to the pyloric sphincter, jejunum 5 cm distal to the ligament of Treitz, ileum 2 cm proximal to the ileocecal junction, and colon 2 cm distal to the cecum.
The segments were placed in a chamber containing ice-cold KBR perfused with carbogen for 30 min.
Removal by dissection of the serosal layer of the duodenal, jejunal, and ileal sections and the serosa and muscularis externa of the colonic sections was performed with the aid of a stereo microscope Wild M 8 and a light source Schott KL 1500.
Intestinal segments rat and human were mounted as flat sheets in a modified Ussing chamber Navicyte; Harvard Apparatus Inc. Some of the reasons for treatment failure are low concentrations of anti-infective agents at the infection site, large numbers of bacteria in the stationary phase, low pH, and high protein content within the abscess 12.Propert, ScD; Anthony J. Alexander, MD; Kathleen J.
In effect, if the drug can penetrate the abscess, its efficacy will be aided by the slower elimination kinetics. In an acute subcutaneous rabbit abscess model, ciprofloxacin was shown to be effective against gram-negative bacilli 4.
More recently, Bamberger et al.